OBTAINING SINGLE DOMAIN NANOBODIES SPECIFIC FOR HUMAN INTERFERON-β AND THEIR USE IN AFFINITY PURIFICATION

Blood of camelids contains both conventional heterotetrameric antibodies and unique functional heavy-chain antibodies. Single-domain antigen-binding fragments of these heavychain antibodies, known as VHH or mini-antibodies (nanobodies) began to be used in basic research. In this review attention is paid to obtaining nanobodies specific for human interferon-β in order to obtain a preparation with high biological activity for application in mass production.