Determination of polyphenolic compounds in raw plant materials by NMR spectroscopy

Environmental monitoring and assessment of the prospects for extracting biologically active substances (BAS) from various types of plant biomass requires the development of simple and fast methods for measuring their content in raw materials. For this purposes mass-spectrometry and NMR spectroscopy are widely used. Both methods have their own advantages and disadvantages. But NMR spectroscopy is a nondestructive method and requires simple sample preparation. In addition, quantitative studies using NMR spectroscopy do not require authentic standard samples. We have developed various techniques for targeted metabolomic analysis of plant origin samples using NMR spectroscopy, including: determination of anthraquinone derivatives in extracts from roots and rhizomes of Rubia tinctorum L., determination of diosmin in Hyssopus officinalis L., determination glucofrangulin A in extracts from leaves of Rhamnus frangula L. © The Authors, published by EDP Sciences.