Разработка и валидация методики количественного определения арбутина в листьях толокнянки обыкновенной (Arctostaphylos uva-ursi (L.) spreng) методом ВЭЖХ/МС

Предложена методика определения арбутина в листьях толокнянки. В качестве метода определения выбран современный, достаточно чувствительный, эффективный метод ВЭЖХ/МС. Подобраны условия, оценены основные метрологические характеристики. Показана высокая эффективность и перспективность разработанной методики, которая может быть рекомендована для определения содержания арбутина в листьях толокнянки в качестве альтернативны существующей фармакопейной титриметрической методике.

The development and validation of the arbutin determination procedure in the bearberry leaves by HPLC/MS

In the article the arbutin determination procedure in the bearberry's leaves is offered. As the method of determination modern, sufficciently sensitive, and effective method HPLC/MS was chosen. The conditions were selected, the main metrological characteristics were estimated. Mass-spectrometric conditions for determination of arbutin at mode multiple reactions monitoring (MRM) were internal standard (phenolphalein), fragmentor's voltage, energy of collision cell, precursor ion, product ion, drying gas temperature - 320°C, pressure gas of nebulaizer - 40 psi, drying gas rate - 10 l/min, capillar's voltage - 4 kV. Conditions of elution were selected: column Protecol C18 HPH125 (250x4,6 mm, 5 pm), gradient, elution rate. Thereby, the procedure of quantitative determination of arbutin in the bearberry's leaves with HPLC/MS was devised. The basic metrological characterisctics of the developed procedure were estimated. It has been shown that HPLC/MS and the developed procedure have high efficiency and prospects and can be recommended for arbutin content determination in medicinal raw plants.