ANALYSIS OF STROMAL CELL CULTURE PROLIFERATION BIOMARKER USING MEDICAL ADHESIVES; АНАЛИЗ БИОМАРКЕРА ПРОЛИФЕРАЦИИ КУЛЬТУРЫ СТРОМАЛЬНЫХ КЛЕТОК ПРИ ИСПОЛЬЗОВАНИИ МЕДИЦИНСКИХ КЛЕЕВ

Introduction: Medical adhesives are widely used in surgical dentistry, including for covering donor sites after free gingival graft harvesting. However, their cytotoxic properties and effects on tissue proliferative activity remain a topic of scientific debate. Objective: To evaluate the effects of various adhesive compositions on the proliferative activity of human oral mucosa stromal cells using Ki-67 staining, and to analyze apoptotic and necrotic changes. Materials and Methods: Three types of medical adhesives were investigated: feracryl-based (Hemocompact), domestic cyanoacrylate (Sulfacrylate), and imported cyanoacrylate (Histoacryl). Adhesives were applied to sterile polycarboxylate plates seeded with human stromal cells. After 24–48 hours of incubation, morphological evaluation, apoptosis and necrosis analysis using Annexin V-FITC/PI, and Ki-67 expression analysis by flow cytometry were per-formed. Statistical analysis included ANOVA and Tukey’s post hoc test (p<0.05). Results: Feracryl samples showed the highest cytotoxicity: elevated necrosis (16.65%) and reduced Ki-67 expression (p<0.001). Cyanoacrylate-based adhesives showed better biocompatibility: Histoacryl demonstrated the lowest necrosis rate and best cell morphology preservation. Both null hypotheses were rejected. Conclusion: Cyanoacrylate-based adhesives, especially imported ones, are safer for use in areas with high regenerative activity. Feracryl-based adhesives are limited due to their significant cytotoxicity. The findings support the use of specific adhesives as wound coverings for donor sites after soft tissue graft harvesting. © 2025 Elsevier B.V., All rights reserved.