Preparation of DNA libraries for sequencingof SAD and FAD genes in flax

Sequencing technologies such as targeted amplicon sequencing are highly effective approaches for studying genetic variation in specific genomic regions as they allow efficient variant identification and characterization. They are useful in studying specific biosynthetic pathways in plants that will allow the breeding of new cultivars with targeted composition. Given that flax seed is a major source of omega-3 fatty acids, we designed a study of the polymorphisms in genes involved in fatty acid biosynthesis. In this study, we report on the library preparation for the targeted amplicon sequencing of these genes.