The variations in gene expression of GAPDH in Ocimum basilicum cultivars under drought-induced stress conditions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) holds a pivotal role within the glycolytic pathway of higher plants.\r\nIt has garnered attention as a signifcant target protein in instances of oxidative stress, where it can engage in thiolation\r\nreactions within its active site. Numerous genes encoding cytosolic iterations of GAPDH have been identifed and analyzed\r\nin specifc plant species. This investigation was conducted to gain insights into GAPDH’s function amidst drought-induced\r\nstress. Within this framework, the basil plant (Ocimum basilicum) was chosen for focused exploration, encompassing the\r\ncloning of the comprehensive cDNA of basil GAPDH (ObGAPDH) and scrutinizing its patterns of expression. The complete\r\nsequence of Ob-GAPDH spanned 1315 base pairs. The resultant protein derived from this sequence comprised 399 amino\r\nacids, projecting a molecular weight of approximately 42.54 kDa and an isoelectric point (pI) of 6.01. An examination of\r\nthe evolutionary connections among various GAPDH proteins unveiled ObGAPDH’s shared lineage with GAPDH proteins\r\nsourced from other plants, such as Salvia splendens and Sesamum indicum. Furthermore, computational methodologies were\r\nharnessed to predict the potential oxidative role of ObGAPDH in response to external signals. Molecular docking simulations illuminated the interaction between ObGAPDH and hydrogen peroxide (H2O2) as a ligand. Scrutinizing the expression\r\npatterns of the ObGAPDH gene under conditions of water scarcity stress brought to light diverse levels of transcriptional\r\nactivity. Collectively, these fndings underscore the notion that the regulation of ObGAPDH expression is contingent upon\r\nboth the specifc plant cultivar and the presence of stress stemming from drought conditions.