The Effects of Interleukin-2 Substances (Glutamate, Glycine, Arginine) on Biofilm Formation in Multidrug-resistant Uropathogenic Escherichia coli in Women with Previous Reproductive Losses

Infectious diseases are very dangerous for the existence of humans and animals. Multidrug-resistant uropathogenic Escherichia coli (UPEC) can cause diseases of the vulva and vagina. The study of uropathogenic E. coli in women who have previously had reproductive losses is relevant. In vitro studies showed that fosfomycin 20mg/disk demonstrated sensitivity in 100 isolates (100%) of uropathogenic multidrug-resistant E. coli. Of the 100 E. coli isolates studied, 40, 37, 31, 29, 21, 19, and 11% were resistant to amikacin (10 μg/disk), ciprofloxacin(30mg/disc), ampicillin (25μg/disc), ceftazidime (20μg/disc), cefuroxime (20mg/disc), ceftriaxone (20μg/disc), ceftazidime and clavulanic acid (10μg/disc), respectively. Whereas 34% were intermediately resistant to gentamicin (10μg/disc). None of the 69 UPEC isolates (69%) tested in women with vaginitis were resistant to Fosfomycin (20μg/disc). The effect of IL-2 and its combination with the enzyme lysozyme and some low-molecular compounds: glutamate, glycine and arginine on clinical isolates of multidrug-resistant UPEC isolated from 100 patients aged 28 to 35 years with laboratory and clinically confirmed nonspecific vaginitis and with previous reproductive losses was studied. The use of recombinant IL-2, α-lysozyme, glutamate, L-glycine, L-arginine at an early stage of UPEC cultivation inhibits biofilms' formation. With the introduction of recombinant IL-2 and glutamine T=24 hours, the biofilm inhibition was 20-30%, 10-15% at T=48 hours and 5-7% at T= 72 hours. The study showed that the bacteriolytic activity of IL-2 against UPEC in women with vaginitis and reproductive losses increases when IL-2 is used in combination with arginine and lysozyme.