Comparative study of phytoplasma DNA extraction methods

The study of phytoplasmas and methods of their detection is an important aspect of determining the phytosanitary condition of the crops in a territory and to help the use of uninfected planting material by agricultural producers. For the study of phytoplasmas it is necessary to carefully and correctly prepare the sample to be analysed with appropriate DNA extraction procedures. Sample preparation consists in extracting fragments of tissues from suitable plant material mainly consisting of phloematic tissues. A comparative study and validation of methods of DNA extraction of phytoplasmas on the example of vegetative parts of the pear tree was carried out. It is shown that the reagent kits CytoSorb and SORB-GMO-B minimize the testing time and are not inferior to the traditional method based on CTAB extraction.