Electron Beam Irradiation Induces Senescence Alterations in Human Adipose-Derived Stem Cells

Cellular and molecular mechanisms underlying aging are currently the subject of increasing interest, with a focus on chronic aseptic nonspecific inflammation linked to aging [1]. Previous studies showed the role of senescence of mesenchymal stem cells secretion in inflammaging, senescent drift induction and aging alterations acceleration [2, 3]. Adipose-Derived Stem Cells (ADSCs) in the human body influences the immune system through both paracrine signaling and direct intercellular interactions. ADSCs have a limited proliferative capacity and are subject to aging, eventually entering a state of senescence. Wide range of cell damage could produce the senescent state of cells, for example by expansion and co-culture. Application of ionizing radiation to investigate cell senescence could mark the renaissance of radiobiology as an approach for disease modeling. The aim of our study was to investigate the effect of fractionated electron irradiation to induce stable senescence in human ADSCs.