INTRODUCTION: Motivation of the young people is a key condition for a full coverage with medical examinations and for a high active detection of sexually transmitted infections (STIs). Modern medical and social conditions dictate the need to update the approaches to educational activities in the prevention of STI. AIM: To determine the effectiveness of preventive measures aimed at motivation to undergo annual screening for detection of STIs and screening in preparation for conception. MATERIALS AND METHODS: A survey of 1,165 students studying in the humanities and technical specialties was conducted. The relationship between preventive measures and the presence of an attitude to undergo examinations was assessed using the relative risk (RR) indicator. RESULTS: The positive influence on motivation for examinations to identify STIs, in the form of routine annual screening and medical preparation for conception was established as a statistically significant direct relationship (RR >1, p ≤0.05) for individual medical consultations, thematic video materials and distance learning on social and psychological aspects. Activities with a statistically significant positive effect in terms of motivation for annual screening (RR >1, p ≤0.05) and a potential effect of explaining medical preparation for parenthood (RR >1, p >0.05) are the inclusion of relevant topics in the educational process and students' educational research work. To note, such forms of preventive work as distant lectures and personal consultations with doctors have a potentially positive effect if the format of their implementation is improved. A negative effect (RR <1) was established in relation to discussion forms of work and existing websites of medical organizations. CONCLUSION: Of priority significance in motivating to STIs screening is direct interaction of young people with employees of medical organizations in the form of individual and group counseling. An important component of effective education is educational and research work. The need to review approaches to presenting information on official websites of medical organizations and limit discussion events has been proven. © 2025 Elsevier B.V., All rights reserved.