ANALYSIS OF PERIODONTAL POCKET MICROBIOTA IN PATIENTS WITH CHRONIC GENERALIZED PERIODONTITIS

Introduction: Periodontal diseases are considered to be among the most prevalent diseases in the maxillofacial region. As indicated by contemporary data, the etiology of inflammatory periodontal diseases is attributed to the accumulation of microbial biofilms on the surfaces of teeth in close proximity to periodontal tissues. The increased activity of periodontopathogenic bacteria can lead to the activation of the body's immune response cascade, which can contribute to the development of gingivitis and periodontitis. The objective of the present study was to investigate the composition of the microbial association of the periodontal pocket in patients exhibiting varying degrees of periodontal tissue damage. Materials and Methods: The microbiologic composition of periodontal pocket contents was studied in a cohort of 34 patients diagnosed with K05.3 Chronic periodontitis. The state of periodontal tissues was evaluated using clinical, instrumental, and radiologic examination methods. The contents of the periodontal pocket were utilized as a subject for examination. The primary identification of microorganisms was carried out by means of the MALDI TOF MS method. Results: The results of the study indicated the presence of various indicators of microbial composition in patients exhibiting different degrees of periodontal tissue lesions. The contents of the periodontal pocket were found to contain both aerobic and anaerobic representatives of bacterial flora. Furthermore, the presence of representatives from the periodontopathogenic spectrum of microorganisms was detected. Conclusions: The microbial composition of the contents of periodontal pockets varies according to the extent of damage to periodontal structures. As the severity of the disease worsened, there was a shift towards an increase in the number of obligate microorganisms and a decrease in species diversity. © 2025 Elsevier B.V., All rights reserved.