One-Pot Time-Induced Proteome Integral Solubility Alteration Assay for Automated and Sensitive Drug-Target Identification

The proteome integral solubility alteration (PISA) assay is widely used for identifying drug targets, but it is labor-intensive and time-consuming and requires a substantial amount of biological sample. Aiming at enabling automation and greatly reducing the sample amount, we developed one-pot time-induced (OPTI)-PISA. Here, we demonstrate OPTI-PISA performance on identifying targets of multiple drugs in cell lysate and scaling down the sample amount to sub-microgram levels, making the PISA method suitable for NanoProteomics. OPTI-PISA can be implemented using only the standard equipment of a proteomics lab. © 2024 Elsevier B.V., All rights reserved.

Авторы
Meng Zhaowei 1, 2, 3 , Saei Amir Ata 4 , Lyu Hezheng 1, 5 , Gaetani Massimiliano 1, 2, 3 , Zubarev Roman A. 1, 2, 3, 6
Журнал
Analytical Chemistry
Издательство
American Chemical Society
Номер выпуска
48
Язык
Английский
Страницы
18917-18921
Статус
Опубликовано
Ссылка
Внешняя ссылка
DOI
10.1021/ACS.ANALCHEM.4C05127
Том
96
Год
2024
Организации
  • 1 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
  • 2 Chemical Proteomics Unit, Science for Life Laboratory, Solna, Sweden
  • 3 Chemical Proteomics, Swedish National Infrastructure for Biological Mass Spectrometry (BioMS), Stockholm, Sweden
  • 4 Department of Microbiology, Karolinska Institutet, Stockholm, Sweden
  • 5 Biomotif AB, Danderyd, Sweden
  • 6 Department of Pharmaceutical and Toxicological Chemistry, RUDN University, Moscow, Russian Federation
Ключевые слова
Targeted drug delivery; Biological samples; Cell lysates; Drug target identification; Drug targets; Labor time; Labour-intensive; One pot; Performance; Scaling down; Standard equipments; Solubility
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