Investigation of the complex antibiotic INA-5812

A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It has been established by MALDI-TOF and ESI-MSn precision mass-spectrometry methods that the main components of the complex antibiotic are several closely related compounds, presumably of a glycopeptide nature. The fraction containing an individual component with a mass of 1845.788 Da has been characterized by UV/Vis absorbance and fluorescence spectra, amino acid analysis, and derivatization with tris(2,6-dimethoxyphenyl)methyl cation. The activity of fractions against pathogenic microbes has been studied. The results allow the supposition that the INA-5812 antibiotic complex is a glyco- or lipoglycopeptide antibiotic of a new type, which is very promising for further study. © 2016, Pleiades Publishing, Ltd.

Авторы
Lapchinskaya O.A. 1 , Katrukha G.S. 1 , Gladkikh E.G. 1 , Kulyaeva V.V. 1 , Coodan P.V. 2 , Topolyan A.P. 3 , Alferova V.A. 1 , Pogozheva V.V. 1 , Sukonnikov M.A. 4 , Rogozhin E.A. 1, 3 , Prokhorenko I.A. 3, 1 , Brylev V.A. 3 , Korolev A.M. 1 , Slyundina M.S. 5, 1 , Borisov R.S. 5, 6 , Serebryakova M.V. 7 , Shuvalov M.V. 4, 1 , Ksenofontov A.L. 7 , Stoyanova L.G. 8 , Osterman I.A. 4, 7 , Formanovsky A.A. 3 , Tashlitsky V.N. 4 , Baratova L.A. 7 , Timofeeva A.V. 7 , Tyurin A.P. 3, 1
Журнал
Russian Journal of Bioorganic Chemistry
Номер выпуска
6
Язык
Английский
Страницы
664-671
Статус
Опубликовано
Ссылка
Внешняя ссылка
DOI
10.1134/S1068162016060078
Том
42
Год
2016
Организации
  • 1 Gause Institute of New Antibiotics, Moscow, 119021, Russian Federation
  • 2 Federal Research Center of Nutrition and Biotechnology, Moscow, 109240, Russian Federation
  • 3 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russian Federation
  • 4 Faculty of Chemistry, Moscow State University, Moscow, 119991, Russian Federation
  • 5 Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, 119991, Russian Federation
  • 6 Faculty of Physics, Mathematics and Natural Sciences, Russian University of Peoples’ Friendship, Moscow, 115419, Russian Federation
  • 7 Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119992, Russian Federation
  • 8 Faculty of Biology, Moscow State University, Moscow, 119991, Russian Federation
Ключевые слова
glycopeptide antibiotics; high performance liquid chromatography; high-resolution mass spectrometry; microbial metabolites
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