Structural and functional study of the receptor binding site for FimH adhesin in uropathogenic strains of Escherichia coli

We evaluated binding capacity of FimH-FocH hybrid adhesins during their interaction with model 1M and 3M substrates and epithelial cells. Introduction of the Glu89Lys point mutation into the filmH gene induced a new 1M-specific phenotype of adhesin. The role of a new pathoadaptive sign in the population of E. coli is discussed.