Isolation and characterization of soluble beta-amyloid and apolipoproteins from cerebrospinal fluid

An original method of isolation and purification of soluble beta-amyloid and apoproteins from the cerebrospinal fluid of healthy donors is developed. The method consists of purification of high density lipoproteins by centrifugation of cerebrospinal fluid and reverse phase high-performance liquid chromatography of isolated lipoproteins. The obtained beta-amyloid and apoproteins from cerebrospinal fluid are characterized immunologically and by mass-spectroscopy.