Background: The current study aimed to develop a laboratory-scale biofilm photobioreactor system for biofuel production.\r\nScope & Approach: During the investigation, Jute was discovered to be the best, cheap, hairy,\r\nopen-pored supporting material for biofilm formation. Microalgae & yeast consortium was used\r\nin this study for biofilm formation.\r\nConclusion: The study identified microalgae and yeast consortium as a promising choice and ideal\r\npartners for biofilm formation with the highest biomass yield (47.63 ± 0.93 g/m2\r\n), biomass\r\nproductivity (4.39 ± 0.29 to 7.77 ± 0.05 g/m2\r\n/day) and lipid content (36%) over 28 days\r\ncultivation period, resulting in a more sustainable and environmentally benign fuel that could\r\nbecome a reality in the near future.